A Silent Death Elie Wiesels Night - 614 Words

â€Å"A silent death, suffocation. No way to scream, to call for help† ( Wiesel 94). Elie Wiesel and many other Jews were put in freight cars and and thought that their lives were over. The jews couldn’t have anyone help them, even when they were calling out for help. Elie wrote a novel called Night, he wrote from his own experience that he had when he was a child. He thought that once he got out of the freight car that it would be the end of the road. At the concentration camps jews could not talk, they had to do everything that Hitler’s men said. While the Holocaust was going on Jews were changing their emotions and how they were acting and working in the camps. When Jews were in the concentration camps they worked non-stop, the Nazis pushed them to keep working hard. The Jews were beaten badly and they got very little food to eat or clothes to wear. If Jews did not do what they were told or talk when not suppose to the Nazis would find a way to punish them. When the Jews were being punished others would not stand up for each other they just stood there silent which gave the Nazis more control over them. â€Å"... he slapped my father with such force that he fell down and then crawled back to his place on all fours† (Wiesel ,39). I think that this is an example of when one Jew is being punished and all the other Jews don’t want to fight the Nazis and save each other. â€Å" I stood petrified. What had happened to me? My father had just been struck, in front of me, and I had not evenShow MoreRelatedEssay on Loss of Religion in Night, by Elie Wiesel1460 Words   |  6 Pagesone-third of the Jewish population were murdered by the Nazis (Holocaust 1). There were many survivors from the holocaust. Elie Wiesel was one of the Jewish people who survived it. He was in three different concentration camps, all of which were horrific. Throughout Elie Wiesel’s novel, Night, Eliezer looses faith in God during and after his tim e in concentration camps. In Night, Eliezer is a Jewish boy who had strong faith in God. He studied the Talmud and spent much time learning about all the differentRead MoreElie Wiesel Night Reflection1035 Words   |  5 Pagesfor its vast dehumanization; nevertheless, history does not acknowledge the faith destroyed as a result. Elie Wiesel, a Nobel Peace Prize winner and author of the memoir Night, recounts his experiences telling his own story of faith being taken away from him. Wiesel, like many other Jews of this time, was taken from his home to Nazi-controlled concentration camps. Before his deportation, Wiesel’s faith used to be an eminent aspect of his life, describing himself as a child of the Talmud; however, theRead MoreNight, Schindlers List, and The Diary of Anne Frank Essay1250 Words   |  5 PagesNight, Schindlers List, and The Diary of Anne Frank The Holocaust was the most horrific time that man has known. To survive this atrocity, the Holocaust victims man upon man atrocity, one had to summon bravery, strength, courage, and wisdom that many did not know they possessed. One survivor is Elie Wiesel, whose exquisite writings have revealed the world of horror suffered by the Jewish people. Elie Wiesels statement, ...to remain silent and indifferent is the greatest sin of all...Read MoreThe World s Leading Spokesman On The Holocaust908 Words   |  4 Pagesbe silent whenever wherever human beings endure suffering and humiliation. We must take sides. Neutrality helps the oppressor, never the victim† (Wiesel par. 9). The inspiring man known as Elie Wiesel was born on September 30, 1928 in Sighet, Romania. He was declared chairman of â€Å"The President’s Commission on the Holocaust†. Wiesel earned the reputation of â€Å"world’s leading spokesman on the Holocaust† because of his extensive discu ssions about the Holocaust and the impact it had on Jews (â€Å"Elie Wiesel-Facts†Read MoreElie Wiesel: A Holocaust Survivor723 Words   |  3 Pagesthat of a witness who believes he has a moral obligation to try to prevent the enemy from enjoying one last victory by allowing his crimes to be erased from human memory† (Wiesel, Night viii). As a result of the horrors that Elie Wiesel experienced during the Holocaust, he devoted his life to become meaningful. Wiesel’s decent disposition changes through atrociously inhumane conduct toward Jews during the Holocaust as he becomes a brute to solidify identity, levy fears, and boost morale. Before hisRead MoreElie Wiesel: Let Us Never Forget1277 Words   |  6 PagesElie Wiesel: Never Forget Elie Wiesel has written over thirty novels over the course of his life. These novels directly affect society in general and especially impact Judaism. He has contributed not only to his race and religion but to ever human soul who reads his work. Elie Wiesel does this by not allowing any to forget the Halocaust of the Jews. Elie Wiesel was born in Signet, Transylvania on September 30, 1928. He grew up the only son of four children, in a close-knit Jewish communityRead MoreThe Holocaust: Night by Elie Wiesel1635 Words   |  7 PagesJews were persecuted, tortured and slaughtered in concentration camps (â€Å"The Holocaust† 1). Night by Elie Wiesel is the powerful memoir of his experiences during the Holocaust. Night shows the tragedy of the Holocaust through the use literary devices, including the themes of loss of faith and cruelty toward other human beings, night as a symbol of suffering and fear, and the use of first person narrative. Night allows the reader to emotionally connect with the victims of the Holocaust, encourages themRead MoreThe Inhumane Treatment Of The Holocaust1714 Words   |  7 Pagessix million of these people being Jewish. Not only were millions murdered, but hundreds of thousands who survived the concentration camps were forever scarred by the dehumanizing events tha t they saw, committed, and lived through. In the novel â€Å"Night† by Elie Wiesel, Wiesel recounts the spine-chillingly horrific events of the Holocaust that affected him first-hand, in an attempt to make the reality of the Holocaust clear and understandable to those who could not believe it. What was arguably one ofRead MoreElie Wiesels Night Essay1159 Words   |  5 PagesElie Wiesels Night As humans, we require basic necessities, such as food, water, and shelter to survive. But we also need a reason to live. The reason could be the thought of a person, achieving some goal, or a connection with a higher being. Humans need something that drives them to stay alive. This becomes more evident when people are placed in horrific situations. In Elie Wiesels memoir Night, he reminisces about his experiences in a Nazi concentration camp during the Holocaust. There theRead MoreNight, By Elie Wiesel1362 Words   |  6 Pagesthe right questions.† (5) In Elie Wiesel s novel â€Å"Night,† we find the horrific life story of a father and son during the period of the devastating Holocaust. Elie and his father need to unite in order to survive through the excruciation. We find a transition of indifference by Elie during his Nobel Peace Prize. From his love for life to not caring about anything, Elie matures as the book progresses. He learns to value his father and stick together. In â€Å"Night,† Elie Weisel utilizes the fear of surviving

Critique of The Stanford Prison Experiment Essay - 757 Words

Critique of The Stanford Prison Experiment The Stanford Prison Experiment of 1973 raises troubling questions about the ability of individuals to exist repressive or obedient roles, if the social setting requires these roles. Philip K. Zimbardo, professor of Psychology at Stanford University, began researching how prisoners and guards assume submissive and authoritarian roles. He set out to do this by placing advertisements in a local newspaper, stating that male college students would be needed for a study of prison life paying fifteen dollars per day for one to two days. Of the seventy-five responses, twenty-one were selected, half of them as guards (Zimbardo p. 364) and the other half as prisoners. (Zimbardo p. 364) Philip†¦show more content†¦In the mock prison, inverse psychological relationships developed between prisoners and guards. Prisoners began to feel that there was no way to beat the system. They felt that it is better to do nothing, except what the guards told them. They didnt want, act, or feel anythin g so they wouldnt get in trouble. Guards, on the other hand, assumed authority roles to control the prisoners and keep the prison in order. Some of the guards reacted extremely, and behaved with hostility and cruelty towards the prisoners. Others, however, were kinder, and occasionally did favors for the prisoners and didnt punish them as much. On the morning of the second day of the experiment, the prisoners broke out in a rebellion. They barricaded themselves in their cells by pushing their cots up against the cell doors; they also proceeded to curse and jeer at the prison guards. The guards regained control of the prison by spraying fire extinguishers on the prisoners and stripping them of their clothing. The guards also forced the leaders of the riot into solitary confinement. Following the riot, the prisoners were more compliant to the rules the guards laid out for them. There was never another united uprising by the prisoners against their authority figures, the guards. After the prisoners had accepted andShow MoreRelatedCritique the Power of Organizations from Weberian and Goffmanesque Perspective in the Stanford Prison Experiment1780 Words   |  8 PagesQ 1. Critique the power of organizations from Weberian and Goffmanesque perspectives in the Stanford Prison This document briefly reviews and critiques the ideas of Weber and Goffman in applying them to the Standard Prison Experiment. Weber identified the significance of bureaucracy within organizations. Within the bureaucratic organization there is a stratification of hierarchy where the legal legitimate authority is invested in individuals who exercise command on the basis of rules andRead MoreSocial Psychology Experiment : The Stanford Prison Experiment967 Words   |  4 Pagessocial psychology experiment, The Stanford Prison Experiment, many have asked whether or not this experiment can really portray how imprisonment can effect an individual. While some say that this experiment is a great representation of the effects caused due to imprisonment, others argue that the experiment was not realistic enough to say it had real effects. Social psychologists Craig Haney and Philip Zimbardo, also the creators of the Stanford Prison Experiment, state that the experiment stimulated aRead MoreCritical View Into Philip Zimbardo s Stanford Prison Experiment 1530 Words   |  7 PagesName: Course Title: Instructor: Submission Date: Critical view into Philip Zimbardo s Stanford Prison Experiment Zimbardo s prison experiment continues to receive a lot of critics from the manner in which it was carried out to the results that its portrayed. Some people believe that as a psychologist, Zimbardo should have done a better job in trying to show how good can turn into evil through the said experiment. The research also demonstrates the interaction between the reality and the illusionRead MoreEssay on Critique of Philip Zimbardos Standfard Prison Experiment1401 Words   |  6 PagesA Critique of Philip Zimbardo’s Prison Experiment â€Å"The Experiment†, an American film in 2010, was directed by Paul Scheuring, and starred by Adrian Brody, 2003 Academy Award’s Best Actor, and Forest Whitaker, a remarkable American actor and director. In the movie, an astounding experiment is conducted by a group of psychological researchers who recruit a group of volunteers to join a prison experiment for cash reward. For two weeks, twenty male participants are hired to play â€Å"prisoners† andRead MoreDD307 TMA062512 Words   |  11 Pagesresearch. The two pieces of research chosen to illustrate this â€Å"Crowds†, the focus of which will be the Stanford prison experiment by Zimbardo (1971) and Professors Haslam and Reicher’s (2002) Prison experiment. Dixon and Mahendran (2012). The second piece of research â€Å"Bystander intervention† focusing on Darley and Latane’s experimental study on the Kitty Genovese murder and Cherry’s feminist critique. Burr (2012) This essay will begin by giving an edited version of Kitty Genovese murder, and why thisRead MoreEssay on Obedience1004 Words   |  5 PagesErin Poulsen ACP W131 Mr. Scanlan 19 October, 2015 Comparative Critique Obedience and Disobedience has been a part of key moments in history. Many have studied forms of obedience to learn how it affects people and situations. For example, Stanley Milgram conducted a well-known experiment in which the subject, named the â€Å"teacher† must shock the â€Å"learner† every time he doesn’t remember a word pair from a memory test. The focus of this study is on the teacher, and whether they will administerRead MoreResearch With Foster Children : Ethical Dilemmas1135 Words   |  5 Pagessensitive issues that come with doing psychological research with human participants. As seen in several famous psychology studies such as the Stanford prison experiment, Milgram experiment, and Tuskegee experiment, ethics in psychological studies are important to protect both the individuals being subjected to research and the researcher. While these specific experiments did not include children, it does bring up an important conversation regarding ethics in research. There are several guidelines put inRead MoreIs Broken Windows A Broken Theory Of Crime?1130 Words   |  5 Pagesminorities. There were two similar experiments that were done: one by the University of Groningen’s social scientist Kees Keizer and his colleagues, and the other by Philip Zimbardo, a psychologist from Stanford. Although the six field experiments done by Kees Keizer and his colleagues did not show that those who committed the crime that were â€Å"artificially created† were white individuals, the experiment done by Zimbardo did (Kelling and Bratton). Zimbardo’s experiment shows that the Broken Windows targetedRead MoreA Critique Of America s Correctional System1444 Words   |  6 PagesA Critique of America’s Correctional System: from prisonization to reform Imprisonment is a rising social issue in America. To put in a numerical perspective, America at only 4% of the world’s population of 7.1 billion is surprisingly the world’s largest jailer at an overwhelming 22 % of the global prison population (Lee 2015). Currently the prison system costs American’s nearly 70 billion dollars (Borowski 2015). With this amount of federal funds going towards housing and feeding inmates, questionsRead MoreHumans : Humans Compassionate Or Aggressive?1880 Words   |  8 Pageshuman species go through all this trouble to prove self- worth? I also believe in the aspect that most people are good natured which would include compassion in their personality. I believe also the idea that Zimbardo, administrator of the Stanford Prison Experiment had which is people can’t be held accountable for their actions it’s the situation This also can be related to the idea aggression only comes out in certain situations in which it is war ranted. The significance of this question can never

Using Waste Cooking Oil as Feedstock and Candida Antarctica Free Essays

Production of Biodiesel by Enzymatic Transesterification: using Waste Cooking Oil as feedstock and Candida Antarctica Lipase B as Biocatalyst. CHAPTER 1 INTRODUCTON The high cost of bio-diesel, compared to petroleum-based diesel, is a major barrier to its commercialization. It has been reported that 60-90% of bio-diesel cost arises from the cost of the feedstock oil (C. We will write a custom essay sample on Using Waste Cooking Oil as Feedstock and Candida Antarctica or any similar topic only for you Order Now C. Lai et al. , 2005). Studies showed the potential of waste-cooking oil (WCO) as a material for biodiesel production (Sulaiman Al-Zuhair, 2008). Therefore, the use of WCO should greatly reduce the cost of bio-diesel. In addition to the choice of lipase employed, factors which make the transesterification process feasible and ready for commercialization are: enzyme modification, the selection of feedstock and alcohol, use of common solvents, pretreatment of the lipase , alcohol to oil molar ratio, water activity/content and reaction temperature. Optimization of these parameters is necessary in order to reduce the cost of biodiesel production. Use of no/low cost waste materials such as the WCO will have double environmental benefits by reducing the environmental pollution potential of the wastes and producing an environmentally friendly fuel. In addition, production of bio-diesel from WCO is considered an important step in reducing and recycling waste. A fresh vegetable oil and its waste differ significantly in water and free fatty acids (FFAs) contents, which are around 2000 ppm and 10-15%, respectively (C. C. Lai et al. , 2005; Y. Zhang et al. , 2003). Because of this the traditional alkaline-catalyzed biodiesel production is unsuitable (Zhang et al. 2003). The use of the enzyme lipase as a biocatalyst for the transesterification reaction step in biodiesel production has been extensively investigated. Lipase is produced by all living organisms and can be used intracellularly or extracellularly. In order to design an economically and environmentally sustainable biodiesel production process, a proper understanding of the f actors affecting the process and their relative importance of enzyme-catalyzed biodiesel production is necessary. A general equation for transesterification (where group R is a fatty acid, R’ is the ength of the acyl acceptor and R† is the rest of the triglyercide molecule) is as follows: Methanol is the most popular alcohol used in the transesterification process because of its relatively cheaper price compared to other alcohols. When methanol is used in the process, the reaction is known as methanolysis as shown in the following equation: Lipases from microorganisms (bacterial and fungal) are the most used as biocatalysts in biotechnological applications and organic chemistry. Fungal – source lipases have been found to produce high yields of lipases compare to the animal and plants. Because their bulk production is easier, commercialization of microbial lipases and their involvement in enzymatic biodiesel production are more common than animal and plant ones (Hasan et al. , 2006;Akoh et al. , 2007; Antczak et al. , 2009). The lipase to be employed as the biocatalyst is Candida Antarctica lipase B (Novozyme CABL L), one of the most common fungal lipase used for the production of biodiesel (Vasudevan and Briggs, 2008). Lipases are capable of converting all the triglycerides derive from the feed stocks into their respective fatty acids methyl esters (FAMEs). They act on the ester bonds of carboxylic acids allowing them to carry out their primary reaction of hydrolyzing fats (Joseph et al. , 2008). Enzyme immobilization is an important approach that could be used as a tool to improve and optimize operation stability, activity and selectivity which allows the enzyme to study under harsher environmental condition and also provides their separation from the reaction mixture without filtration in case of packed bed reactor (Fernandez-Lafuente et al. , 1998; Bhushan et al. , 2009; Gao et al. 2006) and, hence, could lead to more favorable economical benefits. The cost of lipase makes up 90% of the total cost of enzymatic biodiesel production. A significant portion of that is associated with the use of expensive carrier or support materials (Dizge et al. , 2009a). Search for cheaper support materials has been ongoing in order to reduce the overall cost of enzymatic biodiesel production (Robles et al. , 2009). Thus it is important to immobilize l ipase, to be able to recover and reuse it repeatedly ( D. S. Clark,1994;D. Cowan, 1996). Immobilization of lipase is the attachment of the enzyme onto a solid support or the confinement of the enzyme in a region of space (Jegannathan et al. , 2008). When proper strategy for the lipase immobilization technology is employed , it provides a number of important benefits including: (a)enzyme reuse, (b) easy of separation of product from enzyme and (c) the potential to run continuous processes via packed-bed reactors (Peilow and Misbah, 2001). Methods of immobilization include chemical and physical means. Among these, the physical immobilization by way of entrapment is the most widely-used method, in which enzymes are entrapped within the sol-gel matrix prepared by hydrolysis and polycondensation of precursors (Ko Woon Lee, et al. 2010). Tetramethylorthosilicate (TMOS) is a widely used precursor for sol-gel immobilization of the enzyme. However, CALB is unstable and shows low catalytic efficiency in the reaction media contains high concentration of methanol and the lipase is also inhibited by the by-product of glycerol. To overcome this, an amphiphilic matrix is developed to immobilize the lipase ((Ko Woon Lee, et al. 2010). The use of solvent in the transesterification process is also considered. Solvents are used to protect the enzyme from denaturation by alcohol by increasing alcohol solubility (Kumari et al. , 2009). The solvent can also increase the solubility of glycerol which is beneficial since the byproduct can coat the enzyme and inhibit its performance (Royon etal. , 2007 ). The use of a common solvent for the reactants and products not only reduces enzyme inhibition but also ensures a homogeneous reaction mixture, reduces the reaction mixture viscosity and stabilizes the immobilized enzyme (Ranganathan et al. , 2008;Fjerbaek et al. , 2009). This is beneficial because homogeneous reaction mixture decreases problems associated with a multiple phase reaction mixture and a reduced viscosity reduces mass transfer problems around the enzyme (Fjerbaek et al. , 2009). The use of solvents significantly increases the reaction rate in comparison to solvent free systems (Vasudevan and Briggs, 2008). Some study also showed that methanolysis conversion using Candida antarctica was increased when tert-butanol was added to the system (Royon et al. , 2007). This serve as the basis for the choice of tert-butyl to be the solvent use in the system, in order to reduce the inhibition cause of the use of a lower chain alcohol, in this case, the methanol. OBJECTIVES This study aims to produce economical source of feedstocks such as waste-cooking oil for the production of biodiesel and the use of enzyme Candida Antarctica Lipase B, to catalyze to transterification reaction. To be able to determine the yield biodiesel through Gas Chromatographic Analysis (Chrompack CP 9001, Holland). SIGNIFICANCE OF THE STUDY Oil is one of the most commonly reported types of water pollution, causing nearly a quarter of all pollution incidents. Careless disposal of oil into drainage systems, onto land or to watercourses is an offense. It can harm river birds, fish and other wildlife. Because of the way oil spreads, even a small quantity can cause a lot of harm. It is estimated that UK caterers produce between 50 – 90 million litres of waste cooking oil each year. If this is not disposed of correctly the effects of oil pollution on the environment could be quite devastating. According to the Environmental Protection Agency (EPA), estimates that over 200 million gallons of used oil ends up in the trash, and poured into the water each year. This study aims to promote conventional and economic source for the production of biodiesel by using home waste material such as waste cooking oil. Thus, resolving high cost of biodiesel production making it commercially producible and reduce devastation of environment due to high consumption of crude oils from fossil sources. This study will be a significant endeavour in promoting the social needs and to resolve the high prices of the gasoline which is the major economical crisis face in the present society. The advantages of using lipases in biodiesel production are: (a) ability to work in very different media which include biphasic systems, monophasic system (in the presence of hydrophilic or hydrophobic (Am. J. Biochem. Biotech. , 6 (2): 54-76, 2010), (b) they are robust and versatile enzymes that can be produce in bulk because of their extracellular nature in most producing system, (c) many lipases show considerable activity to catalyze transesterification with long or branched chain alcohols, which can hardly be converted to fatty acid esters in the presence of conventional alkaline catalysts, (d) products and byproduct separation in downstream process are xtremely easier, (e) the immobilization of lipases on a carrier has facilitated the repeated use of enzymes after removal from the reaction mixture and when the lipase is in a packed bed reactor, no separation is necessary after transesterification and (f) higher thermostability and short-chain alcohol-tolerant capabilities of lipase make it very convenient for use in biodiesel production (Bacovsky et al. , 2007; Kato et al. , 2007; Robles et al. ,2009). SCOPE AND LIMITATION Like any method for enzymatic biodiesel production, the co st of the lipase to be used is one of great consideration . The limitations of using lipases in biodiesel production include (a) initial activity may be lost because of volume of the oil molecule (Marchetti et al. , 2008; Robles et al. , 2009), (b) the use of solvent does not guarantee the complete protection of enzyme from the inhibitory effect of low chain alcohol, methanol (c) Although lipase is not affected by the high content of FFAs in WCO, the high water content remains a problem (d) the lipase in the biodiesel production is limited on a specific feedstock to be used because of the regioselectivity of the enzyme lipase. CHAPTER 2 REVIEW OF RELATED LITERATURE Biodiesel has shown its ability tomeet the energy demand of the world in the transportation, agriculture, commercial and industrial sectors of the economy (Akoh et al. , 2007; Basha et al. , 2009; Shafiee and Topal, 2009; Robles et al. , 2009). The annual world consumption of diesel is approximately 934 million tons, of which Canada and the United States consume 2. 14 and 19. 06%, respectively (Marchetti et al. , 2008). As a green renewable and potentially unlimited, biodiesel has recently come out as the superlative alternative fuel which can be used in compression ignition engines with minor or no modifications (Xu and Wu, 2003; Vasudevan and Briggs, 2008; Robles et al. , 2009; Leung et al. , 2010). The concept of biofuel is not new. Rudolph Diesel was the first to use a vegetable oil (peanut oil) in a diesel engine in 1911 (Akoh et al. , 2007; Antczak et al. ,2009). The use of biofuels in place of conventional fuels would slow the rogression of global warming by reducing sulfur and carbon oxides and hydrocarbon emissions (Fjerbaek et al. , 2009). Because of economic benefits and more power output, biodiesel is often blended with diesel fuel in ratios of 2, 5 and 20% (Vasudevan and Briggs, 2008). The higher the ratio of biodiesel to diesel the lower the carbon dioxide emission (Fukuda et al. , 2001; Harding et al. , 2007). Using a mixture containing 20% biodiesel reduces carbon dioxide net emissio ns by 15. 66% (Fukuda et al. 2001) while using pure biodiesel makes the net emission of carbon dioxide zero (Vasudevan and Briggs, 2008). The simplest and most efficient route for biodiesel production in large quantities, against less ecofriendly, costly and eventual low yield methods is transesterification. One of the classic organic reactions (transesterification) is the step wise reversible reactions of a triglyceride (fat/oil) with an alcohol to form esters and glycerol. Little excess of alcohol is used to shift the equilibrium towards the formation of esters. Transesterification using an alcohol is a sequence of three reversible consecutive steps. In the first step, triglycerides are converted to diglycerides. In thesecond step, diglycerides are converted to monoglycerides. In the third step, monoglycerides are converted to glycerin molecules (Freedman et al. , 1984; Noureddini and Zhu, 1997; Marchetti et al. , 2008). Each conversion step yields one FAAE molecule, giving a total of three FAAEs per triglyceride molecule as described by the following equations (Murugesan et al. , 2009). 1. Conversion of triglycerides to diglycerides . Conversion of diglycerides to monoglycerides 3. Conversion of monoglycerides tto glycerin molecules In order for the transesterification reaction to be applicable for biodiesel production, the process must be accelerated by the use of catalyst which may be alkaline, acids or enzymes (Bacovsky et al. , 2007; Murugesan et al. ,2009; et al. , 2010). The catalyst employed directly effects the purity of the feedsto ck required, the reaction rate and the extent of post reaction processing needed (McNeff et al. , 2008). To speed up the reaction, heat is also applied. However, this process is very energy intensive and inefficient since FAAE yield below 350 °C is very low and temperatures above 400 °C degrade the ester bonds (Ranganathan et al. , 2008). Generally, the reaction mix is kept just above the boiling point of the alcohol (71-72 °C) to speed up the reaction. The variables known to affect the reaction are: temperature, alcohol to oil molar ratio, catalyst concentration and mixing intensity (Marchetti et al. ,2007). Transesterification catalysts: The transesterification process is catalyzed by alkalis, acids or enzymes. However, the use of alkali catalysts is 100% in commercial sector. The most common alkaline catalysts are sodium hydroxide (NaOH) METHODOLOGY * LIPASE CABL ( Novozyme CABL L) can be purchased from Novozyme (Denmark). All other chemicals can be purchased from Sigma- Aldrich (St. Louis, MO, USA). Grown in the  laboratory,  Candida  appears as large, round, white or cream (albicans  is from  Latin  meaning ‘whitish’) colonies with a yeasty odor on  agar plates  at room temperature. IMMOBILIZATION OF LIPASE Sol – gel immobilization in an amphiphilic matrix was shown in figure below; mL of CABL (8. 2 mg/ml) is to be placed in a 50-ml Falcon tube with 1-mL of 0. 2 M phosphate buffer (pH 7). As a catalyst, 50 microliter of 1M sodium fluoride is to be added and the mixture is to be shaken with a vortex mixer. Then, TMOS (2 mM) and the following hydrophobic alkylsilanes (8 mM) is added; methyltrimethoxysilanes (MTMS), ethyltrimethoxysilane (ETMS), propyltri methoxysilanes (PTMS), and iso-butyltrimethoxysilane (iso-BTMS). Gelation is usually observed within a few minutes while a reaction vessel is gently shaken. Following complete polymerization for 12 hours in a closed Falcon tube, the gel was dried for 24 hours in an open Falcon tube. The gel is to washed with 10 mL of distilled water, 10 mL of 99. 8% iso-propanol, and 10 mL of 95% n-hexane respectively. The immobilized CALB is to be filtered using filter paper, dried at 30 for oC for 36 hours and then ground with mortar and pestle. The particles were sorted using 500 and 300 micrometer sieves and stored at 4 oC until use. ENZYME SOLUTION Immobilized P. cepacia lipase solution is prepare by adding 0. g of lipase to 1 ml of distilled water and soak in water for 30 minutes, prior to being used. This step is found experimentally essential to activate the enzymes. WASTE-COOKING OIL PREPARATION In order to ensure consistency, waste cooking oil is simulated from the commercially available palm oil by heating 1 L of palm oil on a hot plate (Stuart, U. K. ), set at its maximum heating power for two hours. The oil is then allowed to cool to room te mperature and then 5 ml of water (around 5000 ppm) is to be added. The sample is shelved for two weeks before being used. Fresh WCO samples were prepared every two weeks. Bio-Diesel Production in tert-butyl Solvent System Using C. Antarctica Lipase The experiment will be conducted in a specially designed 150 ml capacity jacketed reactor cell. The cell will be kept on a magnetic stirrer (Velp Scientifica, Italy) to facilitate the agitation of the mixture. Water from a temperature controlled water bath (Grant Instruments, UK) circulated through the jacket and will be set to maintain the temperature of the reaction media constant at 45 oC. The temperature used was that presented in the literature to be the optimum(M. M. Soumanou,et al, 2003; H. Fukuda,et al, 2001 ) and an agitation speed was chosen to provide suitable mixing without affecting the activity of the enzyme. In this part, the working volume at the beginning of each experiment was 50 ml, consisting of 5 g of WCO, different volumes of methanol, in the range of 0. 4 to 0. 8 ml (correspond to 0. 57 to 1. 14 molar equivalents of ester bonds in the triglyceride chain), and tert-butyl solution to make up the total volume. The cell is to be covered tightly throughout the progress of the experiments to prevent evaporation. After thermal equilibrium is ensured, 1 ml of enzyme solution containing 0. 4% g of C. Antarctica lipase per g oil is added to initiate the reaction. At suitable intervals, 1. 5 ml samples are withdrawn into a capped vial, immediately immersed in boiling water for at least 5 minutes to denature the enzyme and stop the reaction, and then send for analysis. The amounts of FAMEs in the samples are to be determine by using Gas Chromatograph (Chrompack CP 9001, Holland). How to cite Using Waste Cooking Oil as Feedstock and Candida Antarctica, Essay examples